RT Journal Article SR Electronic T1 NanoDam identifies novel temporal transcription factors conserved between the Drosophila central brain and visual system JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.06.07.447332 DO 10.1101/2021.06.07.447332 A1 Jocelyn L.Y. Tang A1 Anna E. Hakes A1 Robert Krautz A1 Takumi Suzuki A1 Esteban G. Contreras A1 Paul M. Fox A1 Andrea H. Brand YR 2021 UL http://biorxiv.org/content/early/2021/06/08/2021.06.07.447332.abstract AB Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot account for the neuronal diversity in the adult brain. To search for new temporal factors, we developed NanoDam, which enables rapid genome-wide profiling of endogenously-tagged proteins in vivo with a single genetic cross. Mapping the targets of known temporal transcription factors with NanoDam identified Homeobrain and Scarecrow (ARX and NKX2.1 orthologues) as novel temporal factors. We show that Homeobrain and Scarecrow define middle-aged and late INP temporal windows and play a role in cellular longevity. Strikingly, Homeobrain and Scarecrow have conserved functions as temporal factors in the developing visual system. NanoDam enables rapid cell type-specific genome-wide profiling with temporal resolution and can be easily adapted for use in higher organisms.Competing Interest StatementThe authors have declared no competing interest.