PT  - JOURNAL ARTICLE
AU  - Shunji Nakamura
AU  - Tokitaka Oyama
TI  - Adaptive diversification in the cellular circadian behavior of <em>Arabidopsis</em> leaf- and root-derived cells
AID  - 10.1101/2021.06.10.447857
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.06.10.447857
4099  - http://biorxiv.org/content/early/2021/06/10/2021.06.10.447857.short
4100  - http://biorxiv.org/content/early/2021/06/10/2021.06.10.447857.full
AB  - The plant circadian system is based on self-sustained cellular oscillations and is utilized to adapt to daily and seasonal environmental changes. The cellular circadian clocks in the above- and belowground plant organs are subjected to diverse local environments. Individual cellular clocks are affected by other cells/tissues in plants, and the intrinsic properties of cellular clocks remain to be elucidated. In this study, we showed the circadian properties of leaf- and root-derived cells of a CCA1::LUC Arabidopsis transgenic plant and demonstrated that the cells in total isolation from other cells harbor a genuine circadian clock. Quantitative and statistical analyses for individual cellular bioluminescence rhythms revealed a difference in amplitude and precision of light/dark entrainment between the two cell-types, suggesting that leaf-derived cells have a clock with a stronger persistence against fluctuating environments. Circadian systems in the leaves and roots are diversified to adapt to their local environments at the cellular level.Competing Interest StatementThe authors have declared no competing interest.