RT Journal Article SR Electronic T1 Adaptive diversification in the cellular circadian behavior of Arabidopsis leaf- and root-derived cells JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.06.10.447857 DO 10.1101/2021.06.10.447857 A1 Shunji Nakamura A1 Tokitaka Oyama YR 2021 UL http://biorxiv.org/content/early/2021/06/10/2021.06.10.447857.abstract AB The plant circadian system is based on self-sustained cellular oscillations and is utilized to adapt to daily and seasonal environmental changes. The cellular circadian clocks in the above- and belowground plant organs are subjected to diverse local environments. Individual cellular clocks are affected by other cells/tissues in plants, and the intrinsic properties of cellular clocks remain to be elucidated. In this study, we showed the circadian properties of leaf- and root-derived cells of a CCA1::LUC Arabidopsis transgenic plant and demonstrated that the cells in total isolation from other cells harbor a genuine circadian clock. Quantitative and statistical analyses for individual cellular bioluminescence rhythms revealed a difference in amplitude and precision of light/dark entrainment between the two cell-types, suggesting that leaf-derived cells have a clock with a stronger persistence against fluctuating environments. Circadian systems in the leaves and roots are diversified to adapt to their local environments at the cellular level.Competing Interest StatementThe authors have declared no competing interest.