RT Journal Article SR Electronic T1 Estimation of cell cycle kinetics in higher plant root meristem links organ position with cellular fate and chromatin structure JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.01.01.425043 DO 10.1101/2021.01.01.425043 A1 Taras Pasternak A1 Stefan Kircher A1 Klaus Palme YR 2021 UL http://biorxiv.org/content/early/2021/06/13/2021.01.01.425043.abstract AB Plant root development is a complex spatial-temporal process that originates in the root apical meristem (RAM). To shape the organ’s structure signaling between the different cells and cell files must be highly coordinated. Thereby, diverging kinetics of chromatin remodeling and cell growth in these files need to be integrated and balanced by differential cell growth and local differences in cell proliferation frequency. Understanding the local differences in cell cycle duration in the RAM and its correlation with chromatin organization is crucial to build a holistic view on the different regulatory processes and requires a quantitative estimation of the chromatin geometry and underlying mitotic cell cycle phases’ timing at every cell file and every position. Unfortunately, so far precise methods for such analysis are missing.This study presents a robust and straightforward pipeline to determine in parallel the duration of cell cycle’s key stages in all cell layers of a plant’s root and their nuclei organization. The methods combine marker-free techniques based on the detection of the nucleus, deep analysis of the chromatin phase transition, incorporation of 5-ethynyl-2′-deoxyuridine (EdU), and mitosis with a deep-resolution plant phenotyping platform to analyze all key cell cycle events’ kinetics.In the Arabidopsis thaliana L. RAM S-phase duration was found to be as short as 20-30 minutes in all cell files. The subsequent G2-phase duration however depends on the cell type/position and varies from 3.5 hours in the pericycle to more than 4.5 hours in the epidermis. Overall, S+G2+M duration in Arabidopsis under our condition is 4 hours in the pericycle and up to 5.5 hours in the epidermis.Endocycle duration was determined as the time required to achieve 100% EdU index in the transition zone and estimated to be in the range of 3-4 hours.Besides Arabidopsis, we show that the presented technique is applicable also to root tips of other dicot and monocot plants (tobacco (Nicotiana tabacum L.), tomato (Lycopersicon esculentum L.) and wheat (Triticum aestivum L.).Competing Interest StatementThe authors have declared no competing interest.