RT Journal Article
SR Electronic
T1 Distinct gene expression dynamics in developing and regenerating limbs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.14.448408
DO 10.1101/2021.06.14.448408
A1 Chiara Sinigaglia
A1 Alba Almazán
A1 Marie Sémon
A1 Benjamin Gillet
A1 Sandrine Hughes
A1 Eric Edsinger
A1 Michalis Averof
A1 Mathilde Paris
YR 2021
UL http://biorxiv.org/content/early/2021/06/14/2021.06.14.448408.abstract
AB Regenerating animals have the ability to reproduce organs that were originally generated in the embryo and subsequently lost due to injury. Understanding whether the process of regeneration mirrors development is an open question in most regenerative species. Here we take a transcriptomics approach to examine to what extent leg regeneration shows the same temporal patterns of gene expression as leg development in the embryo, in the crustacean Parhyale hawaiensis. We find that leg development in the embryo shows stereotypic temporal patterns of gene expression. In contrast, global patterns of gene expression during leg regeneration show a high degree of variation, related to the physiology of individual animals. A major driver of this variation is the molting cycle. After dissecting the transcriptional signals of individual physiology from regeneration, we obtain temporal signals that mark distinct phases of leg regeneration. Comparing the transcriptional dynamics of development and regeneration we find that, although both processes use largely the same genes, the temporal patterns in which these gene sets are deployed are different and cannot be systematically aligned.HIGHLIGHTSSingle-limb data on transcriptional dynamics of leg development and regenerationDeveloping embryonic legs show stereotypic transcriptional profilesRegenerating leg transcriptomes show a high degree on individual variationRegenerating leg transcriptomes are influenced by adult physiology, especially moltingRegenerating leg transcriptomes reveal distinct phases of leg regenerationLeg development and regeneration use overlapping sets of genes in different temporal patternsCompeting Interest StatementThe authors have declared no competing interest.