RT Journal Article
SR Electronic
T1 Reference-free cell-type deconvolution of pixel-resolution spatially resolved transcriptomics data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.15.448381
DO 10.1101/2021.06.15.448381
A1 Brendan F. Miller
A1 Lyla Atta
A1 Arpan Sahoo
A1 Feiyang Huang
A1 Jean Fan
YR 2021
UL http://biorxiv.org/content/early/2021/06/16/2021.06.15.448381.abstract
AB Recent technological advancements have enabled spatially resolved transcriptomic profiling but at multi-cellular pixel resolution, thereby hindering the identification of cell-type spatial co-localization patterns. We developed STdeconvolve as an unsupervised approach to deconvolve underlying cell-types comprising such multi-cellular pixel resolution spatially resolved transcriptomics datasets. We show that STdeconvolve effectively recovers the putative transcriptomic profiles of cell-types and their proportional representation within spatially resolved pixels without reliance on external single-cell transcriptomics references.Competing Interest StatementThe authors have declared no competing interest.