TY - JOUR T1 - The use of autologous skeletal muscle derived cells as a sling in the treatment of induced stress urinary incontinence, an experimental study JF - bioRxiv DO - 10.1101/2021.06.15.448536 SP - 2021.06.15.448536 AU - Bassem S. Wadie AU - Haytham G. Aamer AU - Sherry M. Khater AU - Mahmoud M. Gabr Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/06/16/2021.06.15.448536.abstract N2 - Introduction & hypothesis This is an experimental pre-clinical study testing for the applicability of autologous skeletal muscle derived cells as a seeded sling for the treatment of SUI in the canine modelMethods 10 Mongrel dogs: In 4, skeletal muscle biopsy was harvested from Biceps Femoris. 1 month later, incontinence was induced in 8 dogs through surgical disruption of the pubourethral ligaments. Muscle biopsy was incubated in DMEM medium and after expansion for 8 weeks, MDCs were collected. PGA scaffold was immersed in culture medium, coated with matrigel and cells were seeded. The sling was placed suburethrally in 8 dogs; 2 of which were cell-seeded and 4 had the scaffold only.Urethral pressure (UP) measurement was done at baseline and 2 weeks alter insertion of the sling.The urethra with its surrounding was harvested 4 weeks after sling insertion for histopathology. 2 dogs were considered as control, in which no urethrolysis or insertion of slings were carried outResults UP show increase of maximum urethral pressure during static measurement in all dogs with a scaffold inserted. The increase ranged from 5-40 cmH20 (Median 23 cmH20)Histopathology shows significant periurethral proliferation of skeletal muscles in 4 dogs with cell-seeded scaffold, as demonstrated by Desmin. This was maximum in dogs # 1& 2. This was not the case in the 4 dogs that had PGA sling only.Conclusion The use of skeletal muscle –seeded PGA scaffold is a practical technique with preserved integrity of histological differentiation in canine model at short term.Brief Summary Autologous Skeletal muscle-derived cells could be propagated in vitro and seeded to PGS scaffolds and used as slingsCompeting Interest StatementThe authors have declared no competing interest. ER -