PT  - JOURNAL ARTICLE
AU  - Wasfi Fares
AU  - Kais Ghedira
AU  - Mariem Gdoura
AU  - Anissa Chouikha
AU  - Sondes Haddad-Boubaker
AU  - Marwa Khedhiri
AU  - Kaouthar Ayouni
AU  - Asma Lamari
AU  - Henda Touzi
AU  - Walid Hammemi
AU  - Zina Medeb
AU  - Amel Sadraoui
AU  - Nahed Hogga
AU  - Nissaf ben Alaya
AU  - Henda Triki
TI  - Sequencing using a two-steps strategy reveals high genetic diversity in the S gene of SARS-CoV-2 after a high transmission period in Tunis, Tunisia
AID  - 10.1101/2021.06.18.449083
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.06.18.449083
4099  - http://biorxiv.org/content/early/2021/06/19/2021.06.18.449083.short
4100  - http://biorxiv.org/content/early/2021/06/19/2021.06.18.449083.full
AB  - Recent efforts have reported numerous variants that influence SARS-CoV-2 viral characteristics including pathogenicity, transmission rate and ability of detection by molecular tests. Whole genome sequencing based on NGS technologies is the method of choice to identify all viral variants; however, the resources needed to use these techniques for a representative number of specimens remain limited in many low and middle income countries. To decrease sequencing cost, we developed a couple of primers allowing to generate partial sequences in the viral S gene allowing rapid detection of numerous variants of concern (VOCs) and variants of interest (VOIs); whole genome sequencing is then performed on a selection of viruses based on partial sequencing results. Two hundred and one nasopharyngeal specimens collected during the decreasing phase of a high transmission COVID-19 wave in T unisia were analyzed. The results reveal high genetic variability within the sequenced fragment and allowed the detection of first introduction in the country of already known VOCs and VOIs as well as others variants that have interesting genomic mutations and need to be kept under surveillance.Importance The method of choice for SARS-CoV-2 variants detection is whole genome sequencing using NGS technologies. Resources for this technology remain limited in many low and middle income countries where it is not possible to perform whole genome sequencing for representative number of SARS-CoV-2 positive cases. In the present work, we developed a novel strategy based on a first partial sanger screening in the S gene including key mutations of the already known VOCs and VOIs for rapid identification of these VOCs and VOIs and helps to better select specimens that need to be sequenced by NGS technologies. The second step consisting in whole genome sequencing allowed to have a holistic view of all variants within the selected viral strains and confirmed the initial classification of the strains based on partial S gene sequencing.