PT - JOURNAL ARTICLE AU - Anindya Ganguly AU - Avinash Chandel AU - Heather Turner AU - Shan Wang AU - Emily R. Liman AU - Craig Montell TI - Requirement for an Otopetrin-Like protein for acid taste in <em>Drosophila</em> AID - 10.1101/2021.06.18.449071 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.06.18.449071 4099 - http://biorxiv.org/content/early/2021/06/19/2021.06.18.449071.short 4100 - http://biorxiv.org/content/early/2021/06/19/2021.06.18.449071.full AB - Many of the Drosophila receptors required for bitter, sugar and other tastes have been identified. However, the receptor required for the taste of acid has been elusive. In Drosophila, the major families of taste receptors, such as “Gustatory Receptors” and “Ionotropic Receptors” are unrelated to taste receptors in mammals. Previous work indicated that members of these major families do not appear to be broadly required acid sensors. Here, to identify the enigmatic acid taste receptor, we interrogated three genes encoding proteins distantly related the mammalian Otopertrin1 proton channel. We found that RNAi knockdown or mutation of Otopetrin-Like A (OtopLA) by CRISPR/Cas9, severely impairs the behavioral rejection of sugary foods laced with HCl or carboxylic acids. Mutation of OtopLA also greatly reduces acid-induced action potentials. We identified an isoform of OtopLA that was expressed in the proboscis and was sufficient to restore acid sensitivity to OtopLA mutant flies. OtopLA functioned in acid taste in a subset of bitter-activated gustatory receptor neurons that senses protons. This work highlights an unusual functional conservation of a receptor required for a taste modality in flies and mammals.Competing Interest StatementThe authors have declared no competing interest.