RT Journal Article
SR Electronic
T1 Mapping the host protein interactome of non-coding regions in SARS-CoV-2 genome
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.19.449092
DO 10.1101/2021.06.19.449092
A1 Liuyiqi Jiang
A1 Mu Xiao
A1 Qing-Qing Liao
A1 Luqian Zheng
A1 Chunyan Li
A1 Yuemei Liu
A1 Bing Yang
A1 Aiming Ren
A1 Chao Jiang
A1 Xin-Hua Feng
YR 2021
UL http://biorxiv.org/content/early/2021/06/19/2021.06.19.449092.abstract
AB A deep understanding of SARS-CoV-2-host interactions is crucial to the development of effective therapeutics. The role of non-coding regions of viral RNA (ncrRNAs) has not been scrutinized. We developed a method using MS2 affinity purification coupled with liquid chromatography-mass spectrometry (MAMS) to systematically map the interactome of SARS-CoV-2 ncrRNA in different human cell lines. Integration of the results defined the core and cell-type-specific ncrRNA-host protein interactomes. The majority of ncrRNA-binding proteins were involved in RNA biogenesis, protein translation, viral infection, and stress response. The 5′ UTR interactome is enriched with proteins in the snRNP family and is a target for the regulation of viral replication and transcription. The 3′ UTR interactome is enriched with proteins involved in the cytoplasmic RNP granule (stress granule) and translation regulation. We show that the ORF10 is likely to be a part of 3′ UTR. Intriguingly, the interactions between negative-sense ncrRNAs and host proteins, such as translation initiation factors and antiviral factors, suggest a pathological role of negative-sense ncrRNAs. Moreover, the cell-type-specific interactions between ncrRNAs and mitochondria may explain the differences of cell lines in viral susceptibility. Our study unveils a comprehensive landscape of the functional SARS-CoV-2 ncrRNA-host protein interactome, providing a new perspective on virus-host interactions and the design of future therapeutics.Competing Interest StatementThe authors have declared no competing interest.