TY - JOUR T1 - The Imaging and Molecular Annotation of Xenografts and Tumours (IMAXT) High Throughput Data and Analysis Infrastructure JF - bioRxiv DO - 10.1101/2021.06.22.448403 SP - 2021.06.22.448403 AU - Eduardo A. González-Solares AU - Ali Dariush AU - Carlos González-Fernández AU - Aybüke Küpcü Yoldaş AU - Mohammad Al Sa’d AU - Neil Millar AU - Tristan Whitmarsh AU - Nicholas Chornay AU - Ilaria Falciatori AU - Atefeh Fatemi AU - Daniel Goodwin AU - Laura Kuett AU - Claire M. Mulvey AU - Marta Páez Ribes AU - Fatime Qosaj AU - Andrew Roth AU - Ignacio Vázquez-García AU - Spencer S. Watson AU - Jonas Windhager AU - Samuel Aparicio AU - Bernd Bodenmiller AU - Ed Boyden AU - Carlos Caldas AU - Owen Harris AU - Sohrab P. Shah AU - Simon Tavaré AU - CRUK IMAXT Grand Challenge Team AU - Dario Bressan AU - Gregory J. Hannon AU - Nicholas A. Walton Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/06/23/2021.06.22.448403.abstract N2 - With the aim of producing a 3D representation of tumours, IMAXT uses a large variety of modalities in order to acquire tumour samples and produce a map of every cell in the tumour and its host environment. With the large volume and variety of data produced in the project we develop automatic data workflows and analysis pipelines and introduce a research methodology where scientists connect to a cloud environment to perform analysis close to where data are located instead of bringing data to their local computers. Here we present the data and analysis infrastructure, discuss the unique computational challenges and describe the analysis chains developed and deployed to generate molecularly annotated tumour models. Registration is achieved by use of a novel technique involving spherical fiducial marks that are visible in all imaging modalities used within IMAXT.Competing Interest StatementSohrab P. Shah is a founder and shareholder of Canexia Health Inc. ER -