RT Journal Article
SR Electronic
T1 Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.06.26.450054
DO 10.1101/2021.06.26.450054
A1 Yuki Sakamoto
A1 Ayako Kawamura
A1 Takamasa Suzuki
A1 Shoji Segami
A1 Masayoshi Maeshima
A1 Stefanie Polyn
A1 Lieven De Veylder
A1 Keiko Sugimoto
YR 2021
UL http://biorxiv.org/content/early/2021/06/27/2021.06.26.450054.abstract
AB Plant cells exhibit remarkable plasticity of their differentiation states, enabling regeneration of whole plants from differentiated somatic cells. How they revert cell fate and express pluripotency, however, remains unclear. Here we show that transcriptional activation of auxin biosynthesis is crucial for reprogramming differentiated Arabidopsis leaf cells. We demonstrate that interfering with the activity of histone acetyltransferases dramatically reduces callus formation from leaf mesophyll protoplasts. Impaired histone acetylation predominantly affects transcription of auxin biosynthesis genes. Auxin biosynthesis is in turn required to accomplish initial cell division through the activation of G2/M phase genes mediated by MYB DOMAIN PROTEIN 3-RELATED (MYB3Rs). We further show that the AUXIN RESPONSE FACTOR 7 (ARF7)/ARF19 and INDOLE-3-ACETIC ACID INDUCIBLE 3 (IAA3)/IAA18-mediated auxin signaling pathway is responsible for cell cycle reactivation in protoplasts. These findings provide novel mechanistic model of how differentiated plant cells can revert their fate and reinitiate the cell cycle to become pluripotent.Competing Interest StatementThe authors have declared no competing interest.