TY - JOUR T1 - Strategies of <em>Mycoplasma ovipneumoniae</em> to evade immune clearance by alveolar macrophages JF - bioRxiv DO - 10.1101/2021.06.28.450257 SP - 2021.06.28.450257 AU - Liyang Gao AU - Kai Zhang AU - Ying Zhang AU - Chunji Ma AU - Xiaoyu Zhou AU - Min Li Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/06/30/2021.06.28.450257.abstract N2 - Chronic nonprogressive pneumonia is a prevalent disease that infects many young sheep. Mycoplasma ovipneumoniae was isolated from the lungs of sheep with chronic nonprogressive pneumonia. Evidences showed that it might associate with the development and duration of chronic pneumonia, moreover, sheep infected with M. ovipneumoniae are easily infected by other organisms, suggesting that M. ovipneumoniae may play an immunosuppressive role during infection. However, the mechanism is still poorly understood. The infection occurs in the airway, where resident alveolar macrophages first encounter M. ovipneumoniae. Therefore, primary alveolar macrophages (AMs) were collected from the lungs of healthy adult sheep, and the (iTRAQ) protein assay was used to investigate the immunosuppressive effects of M. ovipneumoniae on sheep AMs. The RAW264.7 cells were used to confirm the findings. The results showed that M. ovipneumoniae promoted higher expression of anti-apoptotic proteins and lower expression of apoptosis-related proteins in the infected AMs. Moreover, the number of infected AMs increased. However, M. ovipneumoniae reduced ATP levels in AMs and impaired late endosome maturation and phagolysosome fusion. Furthermore, M. ovipneumoniae inhibited the autophagy pathway via the Akt-mTOR axis in AMs. These findings indicated that M. ovipneumoniae had distinctive strategies to evade elimination caused by the AMs. The findings might explain the chronic infection and co-infection in sheep infected by M. ovipneumoniae.Competing Interest StatementThe authors have declared no competing interest.AMsalveolar macrophagesM.ovipneumoniaeMycoplasmaovipneumoniae ER -