PT  - JOURNAL ARTICLE
AU  - Mumbach, Maxwell R.
AU  - Rubin, Adam J.
AU  - Flynn, Ryan A.
AU  - Dai, Chao
AU  - Khavari, Paul A.
AU  - Greenleaf, William J.
AU  - Chang, Howard Y.
TI  - HiChIP: Efficient and sensitive analysis of protein-directed genome architecture
AID  - 10.1101/073619
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 073619
4099  - http://biorxiv.org/content/early/2016/09/08/073619.short
4100  - http://biorxiv.org/content/early/2016/09/08/073619.full
AB  - Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers input requirement over 100-fold relative to ChIA-PET. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to background than in situ Hi-C. Thus, HiChIP adds to the toolbox of 3D genome structure and regulation for diverse biomedical applications.