RT Journal Article
SR Electronic
T1 HiChIP: Efficient and sensitive analysis of protein-directed genome architecture
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 073619
DO 10.1101/073619
A1 Mumbach, Maxwell R.
A1 Rubin, Adam J.
A1 Flynn, Ryan A.
A1 Dai, Chao
A1 Khavari, Paul A.
A1 Greenleaf, William J.
A1 Chang, Howard Y.
YR 2016
UL http://biorxiv.org/content/early/2016/09/08/073619.abstract
AB Genome conformation is central to gene control but challenging to interrogate. Here we present HiChIP, a protein-centric chromatin conformation method. HiChIP improves the yield of conformation-informative reads by over 10-fold and lowers input requirement over 100-fold relative to ChIA-PET. HiChIP of cohesin reveals multi-scale genome architecture with greater signal to background than in situ Hi-C. Thus, HiChIP adds to the toolbox of 3D genome structure and regulation for diverse biomedical applications.