RT Journal Article
SR Electronic
T1 Multifactorial chromatin regulatory landscapes at single cell resolution
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.07.08.451691
DO 10.1101/2021.07.08.451691
A1 Michael P. Meers
A1 Derek H. Janssens
A1 Steven Henikoff
YR 2021
UL http://biorxiv.org/content/early/2021/07/09/2021.07.08.451691.abstract
AB Chromatin profiling at locus resolution uncovers gene regulatory features that define cell types and developmental trajectories, but it remains challenging to map and compare distinct chromatin-associated proteins within the same sample. Here we describe a scalable antibody barcoding approach for profiling multiple chromatin features simultaneously in the same individual cells, Multiple Target Identification by Tagmentation (MulTI-Tag). MulTI-Tag is optimized to retain high sensitivity and specificity of enrichment for multiple chromatin targets in the same assay. We use MulTI-Tag to resolve distinct cell types using multiple chromatin features on a commercial single-cell platform, and to distinguish unique, coordinated patterns of active and repressive element regulatory usage in the same individual cells. Multifactorial profiling allows us to detect novel associations between histone marks in single cells and holds promise for comprehensively characterizing cell-specific gene regulatory landscapes in development and disease.Competing Interest StatementS.H. has filed patent applications related to this work.