PT  - JOURNAL ARTICLE
AU  - Jonathan B. Lynch
AU  - Brittany D. Bennett
AU  - Bryan D. Merrill
AU  - Edward G. Ruby
AU  - Andrew J. Hryckowian
TI  - Independent host- and bacterium-based determinants protect a model symbiosis from phage predation
AID  - 10.1101/2021.07.09.451802
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.09.451802
4099  - http://biorxiv.org/content/early/2021/07/09/2021.07.09.451802.short
4100  - http://biorxiv.org/content/early/2021/07/09/2021.07.09.451802.full
AB  - Bacteriophages (phages) are diverse and abundant constituents of microbial communities worldwide, and are capable of modulating bacterial populations in diverse ways. Here we describe a novel phage, ϕHNL01, which infects the marine bacterium Vibrio fischeri. We use culture-based approaches to demonstrate that mutations in the exopolysaccharide locus of V. fischeri render this bacterium resistant to infection by ϕHNL01, highlighting the extracellular matrix as a key determinant of phage tropism in this interaction. Additionally, using the natural symbiosis between V. fischeri and the squid Euprymna scolopes, we show that during colonization, V. fischeri is protected from phage present in the ambient seawater. Taken together, these findings shed light on independent yet synergistic host- and bacterium-based strategies for resisting symbiosis-disrupting phage predation, and present important implications for understanding these strategies in the context of host-associated microbial ecosystems.Competing Interest StatementThe authors have declared no competing interest.