RT Journal Article
SR Electronic
T1 MAPPING OF LONG-RANGE CHROMATIN INTERACTIONS BY PROXIMITY LIGATION ASSISTED CHIP-SEQ
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 074294
DO 10.1101/074294
A1 Rongxin Fang
A1 Miao Yu
A1 Guoqiang Li
A1 Sora Chee
A1 Tristin Liu
A1 Bing Ren
YR 2016
UL http://biorxiv.org/content/early/2016/09/09/074294.abstract
AB We report a highly sensitive and cost-effective method for genome-wide identification of chromatin interactions in eukaryotic cells. Combining proximity ligation with chromatin immunoprecipitation and sequencing, the method outperforms the state of art approach in sensitivity, accuracy and ease of operation. Application of the method to mouse embryonic stem cells improves mapping of enhancer-promoter interactions.