RT Journal Article SR Electronic T1 Small RNA profiling in Mycobacterium insights into stress adapt ability JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.07.09.451870 DO 10.1101/2021.07.09.451870 A1 Yingyu Chen A1 Wenjun Zhai A1 Kailun Zhang A1 Tingting Zhu A1 Li Su A1 Luiz Bermudez A1 Huanchun Chen A1 Aizhen Guo YR 2021 UL http://biorxiv.org/content/early/2021/07/10/2021.07.09.451870.abstract AB Mycobacteria would encounter a number of environment changes during infection, and respond to it using different mechanisms. sRNA is a posttranscriptionally regulatory system for the function of genes and has been investigated in many other bacteria. Here, we used Mycobacterium tuberculosis and Mycobacterium bovis BCG infection models and sequenced the whole bacterial RNAs before and after host cells infection. Comparison of differential expressed sRNAs, by using GO and KEGG, and target predication, was carried out. Six pathogenically relevant stresses, drug resistance test, growth rate and morphology were used for screening and identify sRNAs. From these data, we identified a subset of sRNAs that are differentially expressed in multiple infection groups and stress conditions. We found that many of them were associated with lipid metabolism. Among them, ncBCG427, was significantly down-regulated when BCG entered into macrophages, and was associated with increase of biofilm formation and changed in drug susceptibility. Then, reduction of virulence possibility depends on regulating lipid metabolism.