PT  - JOURNAL ARTICLE
AU  - Yuting Chen
AU  - Eriona Hysolli
AU  - Anlu Chen
AU  - Stephen Casper
AU  - Songlei Liu
AU  - Kevin Yang
AU  - Chenli Liu
AU  - George Church
TI  - Multiplex base editing to convert TAG into TAA codons in the human genome
AID  - 10.1101/2021.07.13.452007
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.13.452007
4099  - http://biorxiv.org/content/early/2021/07/13/2021.07.13.452007.short
4100  - http://biorxiv.org/content/early/2021/07/13/2021.07.13.452007.full
AB  - Large-scale recoding has been shown to enable novel amino acids, biocontainment and viral resistance in bacteria only so far. Here we extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~ 40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and multiplex editing in mammalian cells.Competing Interest StatementAll G.M.C COI are listed here: http://arep.med.harvard.edu/gmc/tech.html