RT Journal Article
SR Electronic
T1 Substrate, temperature, and geographical patterns among nearly 2,000 natural yeast isolates
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.07.13.452236
DO 10.1101/2021.07.13.452236
A1 William J. Spurley
A1 Kaitlin J. Fisher
A1 Quinn K. Langdon
A1 Kelly V. Buh
A1 Martin Jarzyna
A1 Max A. B. Haase
A1 Kayla Sylvester
A1 Ryan V. Moriarty
A1 Daniel Rodriguez
A1 Angela Sheddan
A1 Sarah Wright
A1 Lisa Sorlie
A1 Amanda Beth Hulfachor
A1 Dana A. Opulente
A1 Chris Todd Hittinger
YR 2021
UL http://biorxiv.org/content/early/2021/07/14/2021.07.13.452236.abstract
AB Yeasts have broad importance as industrially and clinically relevant microbes and as powerful models for fundamental research, but we are only beginning to understand the roles yeasts play in natural ecosystems. Yeast ecology is often more difficult to study compared to other, more abundant microbes, but growing collections of natural yeast isolates are beginning to shed light on fundamental ecological questions. Here we used environmental sampling and isolation to assemble a dataset of 1,962 isolates collected from throughout the contiguous United States of America (USA) and Alaska, which were then used to uncover geographic patterns, along with substrate and temperature associations among yeast taxa. We found some taxa, including the common yeasts Torulaspora delbrueckii and Saccharomyces paradoxus, to be repeatedly isolated from multiple sampled regions of the US, and we classify these as broadly distributed cosmopolitan yeasts. A number of yeast taxon - substrate associations were identified, some of which were novel and some of which support previously reported associations. Further, we found a strong effect of isolation temperature on the phyla of yeasts recovered, as well as for many species. We speculate that substrate and isolation temperature associations reflect the ecological diversity of and niche partitioning by yeast taxa.Take AwayAnalysis of environmental metadata of nearly 2,000 yeast isolates.Individual yeast taxa associate with specific substrates and plant genera.Optimal yeast isolation temperature differs depending on taxonomic rank.Substrate type and isolation temperatures affect isolated yeast diversity.Competing Interest StatementThe authors have declared no competing interest.