RT Journal Article
SR Electronic
T1 Enhancer-promoter interactions and transcription are maintained upon acute loss of CTCF, cohesin, WAPL, and YY1
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.07.14.452365
DO 10.1101/2021.07.14.452365
A1 Tsung-Han S. Hsieh
A1 Claudia Cattoglio
A1 Elena Slobodyanyuk
A1 Anders S. Hansen
A1 Xavier Darzacq
A1 Robert Tjian
YR 2021
UL http://biorxiv.org/content/early/2021/07/14/2021.07.14.452365.abstract
AB It remains unclear why acute depletion of CTCF and cohesin only marginally affects expression of most genes despite substantially perturbing 3D genome folding at the level of domains and structural loops. To address this conundrum, we used high-resolution Micro-C and nascent transcript profiling to find that enhancer-promoter (E-P) interactions are largely insensitive to acute (3-hour) depletion of CTCF, cohesin, and WAPL. YY1 has been proposed to be a structural regulator of E-P loops, but acute YY1 depletion also had minimal effects on E-P loops, transcription, and 3D genome folding. Strikingly, live-cell single-molecule imaging revealed that cohesin depletion reduced transcription factor binding to chromatin. Thus, although neither CTCF, cohesin, WAPL, nor YY1 are required for the short-term maintenance of most E-P interactions and gene expression, we propose that cohesin may serve as a “transcription factor binding platform” that facilitates transcription factor binding to chromatin.Competing Interest StatementThe authors have declared no competing interest.