PT  - JOURNAL ARTICLE
AU  - Sungmin Baek
AU  - Nhung T. T. Tran
AU  - Daniel C. Diaz
AU  - Ya-Yin Tsai
AU  - Tatjana Piotrowski
TI  - High-resolution single cell transcriptome analysis of zebrafish sensory hair cell regeneration
AID  - 10.1101/2021.07.15.452338
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.15.452338
4099  - http://biorxiv.org/content/early/2021/07/15/2021.07.15.452338.short
4100  - http://biorxiv.org/content/early/2021/07/15/2021.07.15.452338.full
AB  - Summary Loss of sensory hair cells in the mammalian inner ear leads to permanent hearing and vestibular defects, whereas loss of hair cells in zebrafish results in their regeneration. We used scRNA-Seq to characterize the transcriptional dynamics of hair cell regeneration in zebrafish at unprecedented spatio-temporal resolution. We uncovered three, sequentially activated modules. First, an injury/inflammatory response and downregulation of progenitor/stem cell maintenance genes within minutes after hair cell loss. Second, the transient activation of regeneration-specific genes. And third, a robust reactivation of developmental gene programs, including hair cell specification, cell cycle activation, ribosome biogenesis, and a metabolic switch to oxidative phosphorylation. The results are not only relevant for our understanding of hair cell regeneration and how we might be able to trigger it in mammals but also for regenerative processes in general. The data is searchable and publicly accessible via a web-based interface.Competing Interest StatementThe authors have declared no competing interest.