RT Journal Article
SR Electronic
T1 Unravelling the puzzle of anthranoids metabolism in living plant cells using spectral imaging coupled to mass spectrometry
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.07.15.452517
DO 10.1101/2021.07.15.452517
A1 Quentin Chevalier
A1 Jean-Baptiste Gallé
A1 Nicolas Wasser
A1 Valérie Mazan
A1 Claire Villette
A1 Jérôme Mutterer
A1 Maria M. Elustondo
A1 Nicolas Girard
A1 Mourad Elhabiri
A1 Hubert Schaller
A1 Andréa Hemmerlin
A1 Catherine Vonthron-Sénécheau
YR 2021
UL http://biorxiv.org/content/early/2021/07/17/2021.07.15.452517.abstract
AB Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from Psorospermum glaberrimum, it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine, emodin, quinizarin as well as lapachol and fraxetin were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoids-derived metabolites and related complexes with cations and proteins. In addition, our study validates the capability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites, leading to new hypotheses on the metabolism of anthranoids in plants.