TY - JOUR T1 - Room Temperature Isothermal Colorimetric Padlock Probe Rolling Circle Amplification for Viral DNA and RNA Detection JF - bioRxiv DO - 10.1101/2020.06.12.128876 SP - 2020.06.12.128876 AU - Wilson Huang AU - Joyce Ting AU - Matthew Fang AU - Hannah Hsu AU - Jimmy Su AU - Tsuyoshi Misaki AU - Derek Chan AU - Justin Yang AU - Ting-Yu Yeh AU - Kelly Yang AU - Vera Chien AU - Tiffany Huang AU - Andrew Chen AU - Claire Wei AU - Jonathan Hsu AU - Jude C. Clapper Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/07/17/2020.06.12.128876.abstract N2 - Seasonal flu and pandemics, which account for millions of infections and hundreds of thousands of deaths, require rapid and reliable detection mechanisms to implement preventive and therapeutic measures. Current detection methods of viral infections have limitations in speed, accuracy, accessibility, and usability. This project presents a novel, widely applicable viral diagnostic test that uses a modified version of rolling circle amplification (RCA) to be sensitive, specific, direct RNA targeted, colorimetric and operable at room temperature. We are specifically detecting the following high-impact viruses: SARS-CoV-2, Influenza A (H1N1pdm09), and Influenza B (Victoria Lineage), although our test can be adapted to any viral infection. Results using synthetic viral DNA and RNA sequences show that our diagnostic test takes approximately one hour, detects femtomolar concentrations of RNA strands, and differentiates between virus strains. We believe implementing our diagnostic test will provide faster responses to future viral-related outbreaks for quicker societal recovery.Competing Interest StatementThe authors have declared no competing interest. ER -