PT - JOURNAL ARTICLE AU - Lucile Fievet AU - Nicolas Serratrice AU - Bénédicte Brulin AU - Laurent Giraudo AU - Julie Véran AU - Nathalie Degardin AU - Florence Sabatier AU - François Féron AU - Pierre Layrolle TI - A comparative <em>in vitro</em> and <em>in vivo</em> study of osteogenicity by using two biomaterials and two human mesenchymal stem cell subtypes AID - 10.1101/2021.07.17.452782 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.07.17.452782 4099 - http://biorxiv.org/content/early/2021/07/19/2021.07.17.452782.short 4100 - http://biorxiv.org/content/early/2021/07/19/2021.07.17.452782.full AB - Bone repair induced by stem cells and biomaterials may represent an alternative to autologous bone grafting. Here, we compared the efficiency of two biomaterials - biphasic calcium phosphate (BCP) and bioactive glass (BG) - when loaded with either adult bone marrow mesenchymal stem cells (BM-MSCs) or newborn nasal ecto-mesenchymal stem cells (NE-MSCs), the latter being collected for further repair of lip cleft-associated bone loss. Both cell types display the typical stem cell surface markers CD73+/CD90+/CD105+/nestin, and exhibit the MSC-associated osteogenic, chondrogenic and adipogenic multipotency. NE-MSCs produce less collagen and alkaline phosphatase than BM-MSCs. At the transcript level, NE-MSCs express more abundantly three genes coding for bone sialoprotein, osteocalcin and osteopontin, while BM-MSCs produce extra copies of RUNX2. BM-MSCs and NE-MSCs adhere and survive on BCP and BG. In vivo experiments reveal that bone formation is only observed with BM-MSCs transplanted on BCP biomaterial.Competing Interest StatementThe authors have declared no competing interest.MSCsmesenchymal stromal stem cellshMSCshuman mesenchymal stem cellsBM-MSCsbone marrow derived MSCsNE-MSCsnasal ecto-mesenchymal stem cellsGMPgood manufacturing practiceBCPbiphasic calcium phosphateHAhydroxyapatiteβTCPbeta-tricalcium phosphateBGbioactive glassPBSphosphate buffered salineCPScalcium phosphate supersaturated solutionPFAparaformaldehydeRTqPCRreal time quantitative PCRGAPDHglyceraldehyde 3-phosphate dehydrogenaseB2Mbeta-2-microglobulinRUNX2RUNX family transcription factor 2BSPbone salioproteinCOLA1collagen A1OCosteocalcinBMP2bone morphogenic protein 2BMP4bone morphogenic protein 4ALPalkaline phosphataseOPosteopontinRTroom temperature