RT Journal Article
SR Electronic
T1 Linking stimuli and behavior with fast near-whole brain recordings in adult Drosophila
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 033803
DO 10.1101/033803
A1 Sophie Aimon
A1 Takeo Katsuki
A1 Logan Grosenick
A1 Michael Broxton
A1 Karl Deisseroth
A1 Terrence J. Sejnowski
A1 Ralph J Greenspan
YR 2016
UL http://biorxiv.org/content/early/2016/09/12/033803.abstract
AB We describe a method to record near-whole brain activity in behaving adult flies. Pan-neuronal calcium and voltage sensors were imaged at high speed with light field microscopy. Functional maps were then extracted by principal component analysis and independent component analysis. Their characteristic shapes match the anatomy of sub-neuropil regions and in some cases a specific neuron type. Responses to light and odor produced activity patterns consistent with previous techniques. Furthermore, the method detected activity linked to behavior as well as a previously uncharacterized pattern of spontaneous activity in the central complex.