PT  - JOURNAL ARTICLE
AU  - Jiaqi Keith Luo
AU  - Holly Melland
AU  - Jess Nithianantharajah
AU  - Sarah L Gordon
TI  - Postsynaptic neuroligin-1 mediates presynaptic endocytosis during neuronal activity
AID  - 10.1101/2021.07.20.453031
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.20.453031
4099  - http://biorxiv.org/content/early/2021/07/20/2021.07.20.453031.short
4100  - http://biorxiv.org/content/early/2021/07/20/2021.07.20.453031.full
AB  - Fast, high-fidelity neurotransmission and synaptic efficacy requires tightly regulated coordination of pre- and postsynaptic compartments and alignment of presynaptic release sites with postsynaptic receptor nanodomains. Neuroligin-1 (Nlgn-1) is a postsynaptic cell-adhesion protein exclusively localised to excitatory synapses that is crucial for coordinating the transsynaptic alignment of presynaptic release sites with postsynaptic AMPA receptors as well as postsynaptic transmission and plasticity. However, little is understood about whether the postsynaptic machinery can mediate the molecular architecture and activity of the presynaptic nerve terminal, and thus it remains unclear whether there are presynaptic contributions to Nlgn1-dependent control of signalling and plasticity. Here, we employed a presynaptic reporter of neurotransmitter release and synaptic vesicle dynamics, synaptophysin-pHluorin (sypHy), to directly assess the presynaptic impact of loss of Nlgn1. We show that lack of Nlgn1 had no effect on the size of the readily releasable or entire recycling pool of synaptic vesicles, nor did it impact exocytosis. However, we observed significant changes in the retrieval of synaptic vesicles by compensatory endocytosis, specifically during activity. Our data extends growing evidence that synaptic adhesion molecules critical for forming transsynaptic scaffolds are also important for regulating activity-induced endocytosis at the presynapse.Competing Interest StatementThe authors have declared no competing interest.