RT Journal Article SR Electronic T1 The Tabula Sapiens: a single cell transcriptomic atlas of multiple organs from individual human donors JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.07.19.452956 DO 10.1101/2021.07.19.452956 A1 The Tabula Sapiens Consortium A1 Quake, Stephen R YR 2021 UL http://biorxiv.org/content/early/2021/07/20/2021.07.19.452956.abstract AB In recent years there has been tremendous progress towards deep molecular characterization of cell types using single cell transcriptome sequencing. Here we report a single cell transcriptomic atlas comprising nearly 500,000 cells from 24 different human tissues and organs. In several instances multiple organs were analyzed from the same donor. Analyzing organs from the same individual controls for genetic background, age, environment, and epigenetic effects, and enables a detailed comparison of cell types that are shared between tissues. This resource provides a rich molecular characterization of more than 400 cell types, their distribution across tissues, and detailed information about tissue specific variation in gene expression. We have used the fact that multiple tissues came from the same donor to study the clonal distribution of T cells between tissues, to understand the tissue specific mutation rate in B cells, and to analyze the cell cycle state and proliferative potential of shared cell types across tissues. Finally, we have also used this data to characterize cell type specific RNA splicing and how such splicing varies across tissues within an individual.One Sentence Summary We used single cell transcriptomics to create a molecularly defined phenotypic reference of human cell types which spans 24 human tissues and organs.Competing Interest StatementThe authors have declared no competing interest.