RT Journal Article
SR Electronic
T1 Rho-dependent control of the Citron kinase, Sticky, drives midbody ring maturation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 566463
DO 10.1101/566463
A1 Nour El-amine
A1 Sabrya C. Carim
A1 Denise Wernike
A1 Gilles R.X. Hickson
YR 2019
UL http://biorxiv.org/content/early/2019/03/03/566463.abstract
AB Rho-dependent proteins control assembly of the cytokinetic contractile ring (CR), yet it remains unclear how those proteins guide ring closure and how they promote subsequent formation of a stable midbody ring (MR). Citron kinase is one important component required for MR formation but its mechanisms of action and relationship with Rho are controversial. Here, we conduct a structure-function analysis of the Drosophila Citron kinase, Sticky, in Schneider’s S2 cells. We define two separable and redundant RhoGEF/Pebble-dependent inputs into Sticky recruitment to the nascent MR and show that each input is subsequently required for retention at, and for the integrity of, the mature MR. The first input is via an actomyosin-independent interaction between Sticky and Anillin, a key scaffold also required for MR formation. The second input requires the Rho-binding domain of Sticky, whose boundaries we have defined. Collectively, these results show how MR biogenesis depends on the coordinated actions of Sticky, Anillin and Rho.