TY - JOUR T1 - Defining the prototypical DNA replication fork trap in bacteria JF - bioRxiv DO - 10.1101/2021.07.20.453168 SP - 2021.07.20.453168 AU - Casey J. Toft AU - Morgane J. J. Moreau AU - Jiri Perutka AU - Savitri Mandapati AU - Peter Enyeart AU - Alanna E. Sorenson AU - Andrew D. Ellington AU - Patrick M. Schaeffer Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/07/22/2021.07.20.453168.abstract N2 - In Escherichia coli, DNA replication termination is orchestrated by two clusters of Ter sites forming a DNA replication fork trap when bound by Tus proteins. The formation of a ‘locked’ Tus-Ter complex is essential for halting incoming DNA replication forks. However, the absence of replication fork arrest at some Ter sites raised questions about their significance. In this study, we examined the genome-wide distribution of Tus and found that only the six innermost Ter sites (TerA-E and G) were significantly bound by Tus. We also found that a single ectopic insertion of TerB in its non-permissive orientation could not be achieved, advocating against a need for ‘back-up’ Ter sites. Finally, examination of the genomes of a variety of Enterobacterales revealed a new replication fork trap architecture exclusively found outside the Enterobacteriaceae family. Taken together, our data enabled the delineation of a narrow prototypical Tus-dependent DNA replication fork trap consisting of only two Ter sites.Competing Interest StatementThe authors have declared no competing interest. ER -