PT  - JOURNAL ARTICLE
AU  - Dylan G. Ryan
AU  - Ming Yang
AU  - Hiran A. Prag
AU  - Giovanny Rodriguez Blanco
AU  - Efterpi Nikitopoulou
AU  - Marc Segarra-Mondejar
AU  - Christopher A. Powell
AU  - Tim Young
AU  - Nils Burger
AU  - Jan Lj. Miljkovic
AU  - Michal Minczuk
AU  - Michael P. Murphy
AU  - Alexander von Kriegsheim
AU  - Christian Frezza
TI  - Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolism
AID  - 10.1101/2021.07.27.453996
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.27.453996
4099  - http://biorxiv.org/content/early/2021/07/27/2021.07.27.453996.short
4100  - http://biorxiv.org/content/early/2021/07/27/2021.07.27.453996.full
AB  - The Tricarboxylic Acid Cycle (TCA) cycle is arguably the most critical metabolic cycle in physiology and exists as an essential interface coordinating cellular metabolism, bioenergetics, and redox homeostasis. Despite decades of research, a comprehensive investigation into the consequences of TCA cycle dysfunction remains elusive. Here, we targeted two TCA cycle enzymes, fumarate hydratase (FH) and succinate dehydrogenase (SDH), and combined metabolomics, transcriptomics, and proteomics analyses to fully appraise the consequences of TCA cycle inhibition (TCAi) in kidney epithelial cells. Our comparative approach shows that TCAi elicits a convergent rewiring of redox and amino acid metabolism dependent on the activation of ATF4 and the integrated stress response (ISR). Furthermore, we also uncover a divergent metabolic response, whereby acute FHi, but not SDHi, can maintain asparagine levels via reductive carboxylation and maintenance of cytosolic aspartate synthesis. Our work highlights an important interplay between the TCA cycle, redox biology and amino acid homeostasis.HighlightsTCA cycle inhibition promotes GSH synthesis and impairs de novo aspartate and proline synthesisDisruption of mitochondrial thiol redox homeostasis phenocopies TCA cycle inhibition by promoting GSH synthesis and impairing proline and aspartate synthesisAcute FHi, but not SDHi, can maintain asparagine levels via reductive carboxylation and maintenance of cytosolic aspartate synthesisTCA cycle inhibition mimics an amino acid deprivation-type response and activates ATF4 via the integrated stress response to maintain redox and amino acid homeostasisCompeting Interest StatementThe authors have declared no competing interest.