PT  - JOURNAL ARTICLE
AU  - Zhong, Yang
AU  - Guo, Siyao
AU  - Ming, Glendon Ong Hong
AU  - Schlundt, Joergen
TI  - The complete genome of carbapenem-resistant <em>Escherichia coli</em> ST410 harbored <em>bla</em><sub>NDM-5</sub> isolated from reservoir water in Singapore
AID  - 10.1101/2021.07.28.454241
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.28.454241
4099  - http://biorxiv.org/content/early/2021/07/29/2021.07.28.454241.short
4100  - http://biorxiv.org/content/early/2021/07/29/2021.07.28.454241.full
AB  - Objective Escherichia coli ST410 with blaNDM-5 has been increasingly detected as multidrug resistance pathogens globally, even though there are very few reports of infections caused by blaNDM-5 producing E. coli in Singapore[1]. And significantly limit sequencing information of blaNDM-5 carried E. coli strain from Singapore. In 2018, our group obtained a carbapenem resistance E. coli ST410 strain SrichA-1 isolated from reservoir water in Singapore, determined to harbor the NDM-5 gene. (BioSample Accession: SAMN18579051).Methods The susceptibility test to antimicrobials was performed with microdilution minimum inhibitory concentration (MIC) test and interpreted according to the Clinical And Laboratory Standards Institute (CLSI) -M100 standards. The genomic DNA of this strain was extracted and send for Whole-genome sequencing(WGS) with the Illumina platform. The WGS analysis was processed with the Center for Genomic Epidemiology (CGE, DTU) server.Results During the minimum inhibitory concentration (MIC) test, SrichA-1 has shown strong resistance to all the beta-lactams, including cephalosporin and carbapenem, which can not be inhibited by the clavulanic acid. Further whole genome sequencing analysis has shown that the strain harboring five beta-lactamase genes covers all class A to D, including the carbapenemase genes as blaNDM-5.Conclusion Here, we reported the complete chromosome sequence of this isolate as well as the sequence of a cycler plasmid. The pSGNDM-5 plasmid was furtherly identified to carry four beta-lactamase genes, including blaNDM-5, blaCTX-M-15, blaTEM-1B, blaOXA-1, while a blaCMY-2 was detected to be located on the chromosome.Competing Interest StatementThe authors have declared no competing interest.