RT Journal Article
SR Electronic
T1 Synaptic plasticity regulated by phosphorylation of PSD-95 Serine 73 in dorsal CA1 is required for contextual fear extinction
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.11.13.381368
DO 10.1101/2020.11.13.381368
A1 Magdalena Ziółkowska
A1 Malgorzata Borczyk
A1 Agata Nowacka
A1 Maria Nalberczak-Skóra
A1 Małgorzata Alicja Śliwińska
A1 Magdalena Robacha
A1 Kacper Łukasiewicz
A1 Anna Cały
A1 Edyta Skonieczna
A1 Kamil F. Tomaszewski
A1 Tomasz Wójtowicz
A1 Jakub Włodarczyk
A1 Tytus Bernaś
A1 Ahmad Salamian
A1 Kasia Radwanska
YR 2021
UL http://biorxiv.org/content/early/2021/07/31/2020.11.13.381368.abstract
AB The ability to extinguish fearful memories is essential for survival. Accumulating data indicate that the dorsal CA1 area (dCA1) contributes to this process. However, the cellular and molecular basis of fear memory extinction remains poorly understood. Postsynaptic density protein 95 (PSD-95) regulates the structure and function of glutamatergic synapses. Here, using dCA1-targeted genetic and chemogenetic manipulations in vivo combined with PSD-95 immunostaining and 3D electron microscopy ex vivo, we demonstrate that phosphorylation of PSD-95 at serine 73 PSD-95(S73) is necessary for contextual fear extinction-induced expression of PSD-95 and synaptic plasticity. Moreover, PSD-95(S73) phosphorylation is not necessary for fear memory formation and recall but is required for extinction of contextual fear. Overall, our data shows how PSD-95-dependent synaptic plasticity in the hippocampus contributes to the persistence of fear memories.Competing Interest StatementThe authors have declared no competing interest.