RT Journal Article
SR Electronic
T1 Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and aging
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.02.454573
DO 10.1101/2021.08.02.454573
A1 Kiara Freitag
A1 Pascale Eede
A1 Andranik Ivanov
A1 Shirin Schneeberger
A1 Tatiana Borodina
A1 Sascha Sauer
A1 Dieter Beule
A1 Frank L. Heppner
YR 2021
UL http://biorxiv.org/content/early/2021/08/04/2021.08.02.454573.abstract
AB Astrocytes are resident glia cells of the central nervous system (CNS) that play complex and heterogeneous roles in brain development, homeostasis and disease. Since their vast involvement in health and disease is becoming increasingly recognized, suitable and reliable tools for studying these cells in vivo and in vitro are of utmost importance. One of the key challenges hereby is to adequately mimic their context-dependent in vivo phenotypes and functions in vitro. To better understand the spectrum of astrocytic variations in defined settings we performed a side-by-side-comparison of embryonic stem cell (ESC)-derived astrocytes as well as primary neonatal and adult astrocytes, revealing major differences on a functional and transcriptomic level, specifically on proliferation, migration, calcium signalling and cilium activity. Our results highlight the need to carefully consider the choice of astrocyte origin and phenotype with respect to age, isolation and culture protocols based on the respective biological question.Competing Interest StatementThe authors have declared no competing interest.