PT  - JOURNAL ARTICLE
AU  - Luke F. Nunnelly
AU  - Melissa Campbell
AU  - Dylan I. Lee
AU  - Guoqiang Gu
AU  - Vilas Menon
AU  - Edmund Au
TI  - St18 specifies globus pallidus projection neuron identity in MGE lineage
AID  - 10.1101/2021.08.03.454907
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.08.03.454907
4099  - http://biorxiv.org/content/early/2021/08/04/2021.08.03.454907.short
4100  - http://biorxiv.org/content/early/2021/08/04/2021.08.03.454907.full
AB  - The medial ganglionic eminence (MGE) is a progenitor domain in the subpallium that produces both locally-projecting interneurons which undergo tangential migration in structures such as the cortex as well as long-range projection neurons that occupy subcortical nuclei. Very little is known about the transcriptional mechanisms specifying the migratory behavior and axonal projection patterns of these two broad classes of MGE-derived neurons. In this study, we identify St18 as a novel transcriptional determinant specifying projection neuron fate in the MGE lineage. St18 is transiently expressed in the MGE subventricular zone (SVZ) and mantle, and we assessed its function using an ES cell-based model of MGE development. Induction of St18 is sufficient to direct ES-derived MGE neurons to adopt a projection neuron-like identity as defined by migration and morphology. Using genetic loss-of-function in mice, we find that St18 is required for the production of globus pallidus pars externa (GPe) prototypic projection neurons. Single cell RNA sequencing revealed that St18 regulates MGE output of specific neuronal populations: in the absence of St18, we observe a large expansion of cortical interneurons at the expense of putative GPe neurons. Through gene expression analysis we identified a downstream effector of St18, Cbx7, which is a component of Polycomb repressor complex 1. We find that Cbx7 is essential for projection neuron-like migration and is not involved in St18-mediated projection neuron-like morphology. Our results characterize a novel transcriptional determinant that directs GPe prototypic projection neuron identity. Further, we identified a downstream target of St18, Cbx7, which regulates only the migratory behavior of long-range projection neurons, suggesting that specific features of MGE projection neuron identity may be governed in a compartmentalized fashion by distinct transcriptional modules downstream of St18.Competing Interest StatementThe authors have declared no competing interest.