RT Journal Article
SR Electronic
T1 A toolbox for studying cortical physiology in primates
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.04.455066
DO 10.1101/2021.08.04.455066
A1 Karam Khateeb
A1 Julien Bloch
A1 Jasmine Zhou
A1 Mona Rahimi
A1 Devon J. Griggs
A1 Viktor N. Kharazia
A1 Min N. Le
A1 Ruikang Wang
A1 Azadeh Yazdan-Shahmorad
YR 2021
UL http://biorxiv.org/content/early/2021/08/05/2021.08.04.455066.abstract
AB Lesioning and neurophysiological studies have facilitated the elucidation of cortical functions and mechanisms of functional recovery following injury. Clinical translation of such studies is contingent on their employment in non-human primates (NHPs), yet tools for monitoring and modulating cortical physiology are incompatible with conventional NHP lesioning techniques. To address these challenges, we developed a toolbox demonstrated in seven macaques. We introduce the photothrombotic method to induce focal cortical lesions alongside a quantitative model for the design of lesion profiles based on experimental needs. Large-scale (∼5 cm2) vascular and neural dynamics can be monitored and lesion induction can be validated in vivo with optical coherence tomography angiography and our electrocorticographic array, the latter of which also enables testing stimulation-based interventions. By combining optical and electrophysiological techniques in NHPs, we can enhance our understanding of cortical functions, investigate functional recovery mechanisms, integrate physiological and behavioral findings, and develop treatments for neurological disorders.Competing Interest StatementRKW discloses intellectual property owned by the Oregon Health and Science University and the University of Washington. He is a consultant to Carl Zeiss Meditec. The remaining authors declare no disclosures.