TY - JOUR T1 - Paracrine granules are cytoplasmic RNP granules distinct from stress granules that assemble in response to viral infection JF - bioRxiv DO - 10.1101/2021.08.06.455464 SP - 2021.08.06.455464 AU - Valentina Iadevaia AU - James M. Burke AU - Lucy Eke AU - Carla Moller-Levet AU - Roy Parker AU - Nicolas Locker Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/08/06/2021.08.06.455464.abstract N2 - To rapidly respond and adapt to stresses, such as viral infections, cells have evolved several mechanisms, which include the activation of stress response pathways and the innate immune response. These stress responses result in the rapid inhibition of translation and condensation of stalled mRNAs, together with RNA-binding proteins and signalling components, into cytoplasmic biocondensates called stress granules. Increasing evidence suggests that stress granules contribute to antiviral defense and thus viruses need to evade these response pathways to propagate. In addition, the stress granule pathway is proposed to be dynamic and adaptable to specific stresses. We previously showed that Feline Calicivirus (FCV) impairs SGs assembly by cleaving the scaffolding protein G3BP1. We also observed that uninfected bystander cells assembled G3BP1-granules, suggesting a paracrine response trigged by the infection. We now present evidence that virus-free supernatant generated from infected cells can induce the formation of paracrine granules. They are different from canonical stress granules and exhibit specific kinetics of assembly-disassembly, protein and RNA composition and are linked to antiviral activity. We propose that this paracrine induction reflects a novel cellular defence mechanism to limit viral propagation and promote stress responses in bystander cells.Summary statement We describe a novel type of paracrine induced RNA granules associated with viruses, highlighting how different stresses results in heterogeneous stress granule-like condensates with specific cellular functions.Competing Interest StatementThe authors have declared no competing interest. ER -