RT Journal Article
SR Electronic
T1 Large field-of-view incoherent volumetric imaging in living human retina by confocal oblique scanning laser ophthalmoscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.05.455286
DO 10.1101/2021.08.05.455286
A1 Wenjun Shao
A1 Ji Yi
YR 2021
UL http://biorxiv.org/content/early/2021/08/07/2021.08.05.455286.abstract
AB Three-dimensional (3D) volumetric imaging of the human retina is instrumental to monitor and diagnose blinding conditions. Although coherent retinal imaging is well established by optical coherence tomography, it is still a large void for incoherent volumetric imaging in the human retina. Here, we report confocal oblique scanning laser ophthalmoscopy (CoSLO), to fill that void and harness incoherent optical contrast in 3D. CoSLO uses oblique scanning laser and remote focusing to acquire depth signal in parallel, avoid the lengthy z-stacking, and image a large field of view (FOV). In addition, confocal gating is introduced by a linear sensor array to improve the contrast and resolution. For the first time, we achieved incoherent 3D human retinal imaging with >20° viewing angle within only 5 seconds. The depth resolution is ∼45 microns in vivo. We demonstrated label-free incoherent contrast by CoSLO, revealing unique features in the retina. CoSLO will be an important technique for clinical care of retinal conditions and fundamental vision science, by offering unique volumetric incoherent contrasts.Competing Interest StatementThe authors have declared no competing interest.