PT  - JOURNAL ARTICLE
AU  - MICrONS Consortium
AU  - J. Alexander Bae
AU  - Mahaly Baptiste
AU  - Agnes L. Bodor
AU  - Derrick Brittain
AU  - JoAnn Buchanan
AU  - Daniel J. Bumbarger
AU  - Manuel A. Castro
AU  - Brendan Celii
AU  - Erick Cobos
AU  - Forrest Collman
AU  - Nuno Maçarico da Costa
AU  - Sven Dorkenwald
AU  - Leila Elabbady
AU  - Paul G. Fahey
AU  - Tim Fliss
AU  - Emmanouil Froudarakis
AU  - Jay Gager
AU  - Clare Gamlin
AU  - Akhilesh Halageri
AU  - James Hebditch
AU  - Zhen Jia
AU  - Chris Jordan
AU  - Daniel Kapner
AU  - Nico Kemnitz
AU  - Sam Kinn
AU  - Selden Koolman
AU  - Kai Kuehner
AU  - Kisuk Lee
AU  - Kai Li
AU  - Ran Lu
AU  - Thomas Macrina
AU  - Gayathri Mahalingam
AU  - Sarah McReynolds
AU  - Elanine Miranda
AU  - Eric Mitchell
AU  - Shanka Subhra Mondal
AU  - Merlin Moore
AU  - Shang Mu
AU  - Taliah Muhammad
AU  - Barak Nehoran
AU  - Oluwaseun Ogedengbe
AU  - Christos Papadopoulos
AU  - Stelios Papadopoulos
AU  - Saumil Patel
AU  - Xaq Pitkow
AU  - Sergiy Popovych
AU  - Anthony Ramos
AU  - R. Clay Reid
AU  - Jacob Reimer
AU  - Casey M. Schneider-Mizell
AU  - H. Sebastian Seung
AU  - Ben Silverman
AU  - William Silversmith
AU  - Amy Sterling
AU  - Fabian H. Sinz
AU  - Cameron L. Smith
AU  - Shelby Suckow
AU  - Marc Takeno
AU  - Zheng H. Tan
AU  - Andreas S. Tolias
AU  - Russel Torres
AU  - Nicholas L. Turner
AU  - Edgar Y. Walker
AU  - Tianyu Wang
AU  - Grace Williams
AU  - Sarah Williams
AU  - Kyle Willie
AU  - Ryan Willie
AU  - William Wong
AU  - Jingpeng Wu
AU  - Chris Xu
AU  - Runzhe Yang
AU  - Dimitri Yatsenko
AU  - Fei Ye
AU  - Wenjing Yin
AU  - Szi-chieh Yu
TI  - Functional connectomics spanning multiple areas of mouse visual cortex
AID  - 10.1101/2021.07.28.454025
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.07.28.454025
4099  - http://biorxiv.org/content/early/2021/08/09/2021.07.28.454025.short
4100  - http://biorxiv.org/content/early/2021/08/09/2021.07.28.454025.full
AB  - The value of an integrated approach for understanding the neocortex by combining functional characterization of single neuron activity with the underlying circuit architecture has been understood since the dawn of modern neuroscience. However, in practice, anatomical connectivity and physiology have been studied mostly separately. Following in the footsteps of previous studies that have combined physiology and anatomy in the same tissue, here we present a unique functional connectomics dataset that contains calcium imaging of an estimated 75,000 neurons from primary visual cortex (VISp) and three higher visual areas (VISrl, VISal and VISlm), that were recorded while a mouse viewed natural movies and parametric stimuli. The functional data were co-registered with electron microscopy (EM) data of the same volume which were automatically segmented, reconstructing more than 200,000 cells (neuronal and non-neuronal) and 524 million synapses. Subsequent proofreading of some neurons in this volume yielded reconstructions that include complete dendritic trees as well the local and inter-areal axonal projections. The largest proofread excitatory axon reached a length of 19 mm and formed 1893 synapses, while the largest inhibitory axon formed 10081 synapses. Here we release this dataset as an open access resource to the scientific community including a set of analysis tools that allows easy data access, both programmatically and through a web user interface.Competing Interest StatementTM and HSS disclose financial interests in Zetta AI LLC. JR and AST disclose financial interests in Vathes LLC.