RT Journal Article SR Electronic T1 Functional connectomics spanning multiple areas of mouse visual cortex JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.07.28.454025 DO 10.1101/2021.07.28.454025 A1 MICrONS Consortium A1 J. Alexander Bae A1 Mahaly Baptiste A1 Agnes L. Bodor A1 Derrick Brittain A1 JoAnn Buchanan A1 Daniel J. Bumbarger A1 Manuel A. Castro A1 Brendan Celii A1 Erick Cobos A1 Forrest Collman A1 Nuno Maçarico da Costa A1 Sven Dorkenwald A1 Leila Elabbady A1 Paul G. Fahey A1 Tim Fliss A1 Emmanouil Froudarakis A1 Jay Gager A1 Clare Gamlin A1 Akhilesh Halageri A1 James Hebditch A1 Zhen Jia A1 Chris Jordan A1 Daniel Kapner A1 Nico Kemnitz A1 Sam Kinn A1 Selden Koolman A1 Kai Kuehner A1 Kisuk Lee A1 Kai Li A1 Ran Lu A1 Thomas Macrina A1 Gayathri Mahalingam A1 Sarah McReynolds A1 Elanine Miranda A1 Eric Mitchell A1 Shanka Subhra Mondal A1 Merlin Moore A1 Shang Mu A1 Taliah Muhammad A1 Barak Nehoran A1 Oluwaseun Ogedengbe A1 Christos Papadopoulos A1 Stelios Papadopoulos A1 Saumil Patel A1 Xaq Pitkow A1 Sergiy Popovych A1 Anthony Ramos A1 R. Clay Reid A1 Jacob Reimer A1 Casey M. Schneider-Mizell A1 H. Sebastian Seung A1 Ben Silverman A1 William Silversmith A1 Amy Sterling A1 Fabian H. Sinz A1 Cameron L. Smith A1 Shelby Suckow A1 Marc Takeno A1 Zheng H. Tan A1 Andreas S. Tolias A1 Russel Torres A1 Nicholas L. Turner A1 Edgar Y. Walker A1 Tianyu Wang A1 Grace Williams A1 Sarah Williams A1 Kyle Willie A1 Ryan Willie A1 William Wong A1 Jingpeng Wu A1 Chris Xu A1 Runzhe Yang A1 Dimitri Yatsenko A1 Fei Ye A1 Wenjing Yin A1 Szi-chieh Yu YR 2021 UL http://biorxiv.org/content/early/2021/08/09/2021.07.28.454025.abstract AB The value of an integrated approach for understanding the neocortex by combining functional characterization of single neuron activity with the underlying circuit architecture has been understood since the dawn of modern neuroscience. However, in practice, anatomical connectivity and physiology have been studied mostly separately. Following in the footsteps of previous studies that have combined physiology and anatomy in the same tissue, here we present a unique functional connectomics dataset that contains calcium imaging of an estimated 75,000 neurons from primary visual cortex (VISp) and three higher visual areas (VISrl, VISal and VISlm), that were recorded while a mouse viewed natural movies and parametric stimuli. The functional data were co-registered with electron microscopy (EM) data of the same volume which were automatically segmented, reconstructing more than 200,000 cells (neuronal and non-neuronal) and 524 million synapses. Subsequent proofreading of some neurons in this volume yielded reconstructions that include complete dendritic trees as well the local and inter-areal axonal projections. The largest proofread excitatory axon reached a length of 19 mm and formed 1893 synapses, while the largest inhibitory axon formed 10081 synapses. Here we release this dataset as an open access resource to the scientific community including a set of analysis tools that allows easy data access, both programmatically and through a web user interface.Competing Interest StatementTM and HSS disclose financial interests in Zetta AI LLC. JR and AST disclose financial interests in Vathes LLC.