RT Journal Article SR Electronic T1 OPAM, an Open source, 3D printed Low-cost Micro-Manipulator for Single Cell Manipulation JF bioRxiv FD Cold Spring Harbor Laboratory SP 2021.08.09.455588 DO 10.1101/2021.08.09.455588 A1 Jiang Xu A1 Zhuowei Du A1 Paul Liu A1 Yi Kou A1 Lin Chen YR 2021 UL http://biorxiv.org/content/early/2021/08/10/2021.08.09.455588.abstract AB We introduce OPAM, an Open source, low-cost (under $150), 3D-Printed, stepper motor driven, Arduino based, single cell Micromanipulator (OPAM). Modification of a commercial stepper motor led to dramatically increased stability and maneuverability of the motor, based on which the micromanipulator was designed. All components of this micromanipulator can be 3D printed using an entry-level 3D printer and assembled with ease. With this single cell manipulator, successful targeted single cell capture and transfer was confirmed under the microscope, which showed great promise for single cell related experiments.Competing Interest StatementThe authors have declared no competing interest.