PT - JOURNAL ARTICLE AU - Davis, Amanda M. AU - Iovinella, Manuela AU - James, Sally AU - Robshaw, Thomas AU - Dodson, Jennifer R. AU - Herrero-Davila, Lorenzo AU - Clark, James H. AU - Agapiou, Maria AU - McQueen-Mason, Simon J. AU - Pinto, Gabriele AU - Ciniglia, Claudia AU - Chong, James P. J. AU - Ashton, Peter D. AU - Davis, Seth J. TI - Using MinION nanopore sequencing to generate a <em>de novo</em> eukaryotic draft genome: preliminary physiological and genomic description of the extremophilic red alga <em>Galdieria sulphuraria</em> strain SAG 107.79 AID - 10.1101/076208 DP - 2016 Jan 01 TA - bioRxiv PG - 076208 4099 - http://biorxiv.org/content/early/2016/09/20/076208.short 4100 - http://biorxiv.org/content/early/2016/09/20/076208.full AB - We report here the de novo assembly of a eukaryotic genome using only MinION nanopore DNA sequence data by examining a novel Galdieria sulphuraria genome: strain SAG 107.79. This extremophilic red alga was targeted for full genome sequencing as we found that it could grow on a wide variety of carbon sources and could uptake several precious and rare-earth metals, which places it as an interesting biological target for disparate industrial biotechnological uses. Phylogenetic analysis clearly places this as a species of G. sulphuraria. Here we additionally show that the genome assembly generated via nanopore long read data was of a high quality with regards to low total number of contiguous DNA sequences and long length of assemblies. Collectively, the MinION platform looks to rival other competing approaches for de novo genome acquisition with available informatics tools for assembly. The genome assembly is publically released as NCBI BioProject PRJNA330791. Further work is needed to reduce small insertion-deletion errors, relative to short-read assemblies.