TY - JOUR T1 - Temporal properties of painful contrast enhancement using repetitive stimulation JF - bioRxiv DO - 10.1101/2021.08.12.456139 SP - 2021.08.12.456139 AU - Tibor M. Szikszay AU - Waclaw M. Adamczyk AU - Juliette L. M. Lévénez AU - Philip Gouverneur AU - Kerstin Luedtke Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/08/13/2021.08.12.456139.abstract N2 - Offset analgesia is characterized by a disproportionately large reduction in pain following a small decrease in a heat stimulus and is based on the phenomenon of temporal pain contrast enhancement (TPCE). The aim of this study is to investigate whether this phenomenon can also be induced by repetitive stimulation, i.e., by stimuli that are clearly separated in time. With this aim, the repetitive TPCE paradigm was induced in healthy, pain-free subjects (n=33) at the volar non-dominant forearm using heat stimuli. This paradigm was performed applying three different interstimulus intervals (ISIs): 5, 15, and 25 seconds. All paradigms were contrasted with a control paradigm without temperature change. Participants continuously rated the perceived pain intensity. In addition, electrodermal activity was recorded as a surrogate measure of autonomic arousal. Temporal pain contrast enhancement was confirmed for both ISI 5 seconds (p < 0.001) and ISI 15 seconds (p = 0.005), but not for ISI 25 seconds (p = 0.07), however the magnitude of TPCE did not differ between ISIs (p = 0.11). Electrodermal activity was consistent previous pain ratings, but showing significantly higher autonomic activity being measured. Thus, the phenomenon of temporal contrast enhancement of pain can also be induced by repetitive stimulation. Both the involvement of the autonomic nervous system and the involvement of habituation processes are conceivable, which consequently points to both central and peripheral mechanisms of TPCE.Summary The temporal contrast enhancement of pain and electrodermal activity can be provoked by stimuli that are clearly separated in time.Competing Interest StatementThe authors have declared no competing interest. ER -