PT  - JOURNAL ARTICLE
AU  - Alexandre P. Thiery
AU  - Ariane S. I. Standing
AU  - Rory L. Cooper
AU  - Gareth J. Fraser
TI  - An ancient dental signalling centre supports homology of an enamel knot in sharks
AID  - 10.1101/2021.08.13.456270
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.08.13.456270
4099  - http://biorxiv.org/content/early/2021/08/16/2021.08.13.456270.short
4100  - http://biorxiv.org/content/early/2021/08/16/2021.08.13.456270.full
AB  - Development of tooth cusps is regulated by the enamel knot signalling centre. Fgf signalling regulates differential proliferation between the enamel knot and adjacent dental epithelia during tooth development, leading to formation of the dental cusp. The presence of an enamel knot in non-mammalian vertebrates is debated given differences in signalling. Here we show the conservation and restriction of fgf10 and fgf3 to the sites of future dental cusps in the shark (Scyliorhinus canicula), whilst also highlighting striking differences between the shark and mouse. We reveal shifts in tooth size, shape and cusp number following small molecule perturbations of canonical Wnt signalling. Resulting tooth phenotypes mirror observed effects in mammals, where canonical Wnt has been implicated as an upstream regulator of enamel knot signalling. In silico modelling of shark dental morphogenesis demonstrates how subtle changes in activatory and inhibitory signals can alter tooth shape, resembling phenotypes observed following experimental Wnt perturbation. Our results support the functional conservation of an enamel knot-like signalling centre throughout vertebrates and suggest that varied tooth types from sharks to mammals follow a similar developmental bauplan. Lineage-specific differences in signalling are not sufficient in refuting homology of this signalling centre, which is likely older than teeth themselves.Competing Interest StatementThe authors have declared no competing interest.