PT  - JOURNAL ARTICLE
AU  - S. Wunderl
AU  - A. Ishijima
AU  - E. A. Susaki
AU  - Z. Xu
AU  - H. Song
AU  - H. Zha
AU  - T. Azuma
AU  - I. Sakuma
AU  - H. Fukuoka
AU  - E. Okada
AU  - H. R. Ueda
AU  - S. Takagi
AU  - K. Nakagawa
TI  - Acoustic light-sheet microscopy
AID  - 10.1101/2021.08.20.457051
DP  - 2021 Jan 01
TA  - bioRxiv
PG  - 2021.08.20.457051
4099  - http://biorxiv.org/content/early/2021/08/20/2021.08.20.457051.short
4100  - http://biorxiv.org/content/early/2021/08/20/2021.08.20.457051.full
AB  - Light-sheet imaging of 3D objects with high spatial resolution remains an open challenge because of the trade-off between field-of-view (FOV) and axial resolution originating from the diffraction of light. We developed acoustic light-sheet microscopy (acoustic LSM), which actively manipulates the light propagation inside a large sample to obtain wide-field microscopic images deep inside a target. By accurately coupling a light-sheet illumination pulse into a planar acoustic pulse, the light-sheet can be continuously guided over large distances. We imaged a fluorescence-labeled transparent mouse brain for the FOVs of 19.3 × 12.4 mm2 and 9.7 × 5.9 mm2 with resolved microstructures and single cells deep inside the brain. Acoustic LSM creates new opportunities for the application of light-sheet in the field of industry to basic science.One Sentence Summary An acoustic-optical method overcomes a trade-off between field-of-view and axial resolution in light-sheet microscopy.Competing Interest StatementThe authors have declared no competing interest.