TY - JOUR T1 - Plasma Contains Ultra-short Single-stranded DNA in Addition to Nucleosomal cfDNA JF - bioRxiv DO - 10.1101/2021.08.22.457243 SP - 2021.08.22.457243 AU - Jordan Cheng AU - Marco Morselli AU - Wei-Lun Huang AU - You Jeong Heo AU - Thalyta Pinheiro-Ferreira AU - Feng Li AU - Fang Wei AU - David Chia AU - Hua Jun AU - Kenneth Cole AU - Wu-Chou Su AU - Matteo Pellegrini AU - David TW Wong Y1 - 2021/01/01 UR - http://biorxiv.org/content/early/2021/08/24/2021.08.22.457243.abstract N2 - Plasma cell-free DNA is a widely used biomarker for diagnostic screening. We introduce uscfDNA-seq, a single-stranded cell-free DNA NGS pipeline, which bypasses previous limitations to reveal a novel population of ultrashort single-stranded cell-free DNA in plasma with a modal size of 50nt. This species of cfDNA aligns predominantly to the nuclear genome and could potentially be used for novel biomarker discovery.Competing Interest StatementDavid Wong is a consultant to GSK, Mars-Wrigley, Colgate Palmolive and has equity in Liquid Diagnostics LLC. ER -