RT Journal Article
SR Electronic
T1 Plasma Contains Ultra-short Single-stranded DNA in Addition to Nucleosomal cfDNA
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.08.22.457243
DO 10.1101/2021.08.22.457243
A1 Jordan Cheng
A1 Marco Morselli
A1 Wei-Lun Huang
A1 You Jeong Heo
A1 Thalyta Pinheiro-Ferreira
A1 Feng Li
A1 Fang Wei
A1 David Chia
A1 Hua Jun
A1 Kenneth Cole
A1 Wu-Chou Su
A1 Matteo Pellegrini
A1 David TW Wong
YR 2021
UL http://biorxiv.org/content/early/2021/08/24/2021.08.22.457243.abstract
AB Plasma cell-free DNA is a widely used biomarker for diagnostic screening. We introduce uscfDNA-seq, a single-stranded cell-free DNA NGS pipeline, which bypasses previous limitations to reveal a novel population of ultrashort single-stranded cell-free DNA in plasma with a modal size of 50nt. This species of cfDNA aligns predominantly to the nuclear genome and could potentially be used for novel biomarker discovery.Competing Interest StatementDavid Wong is a consultant to GSK, Mars-Wrigley, Colgate Palmolive and has equity in Liquid Diagnostics LLC.