PT - JOURNAL ARTICLE AU - Arnoud H.M. van Vliet AU - Siddhartha Thakur AU - Joaquin M. Prada AU - Jai W. Mehat AU - Roberto M. La Ragione TI - Genomic screening of antimicrobial resistance markers in UK and US <em>Campylobacter</em> isolates highlights stability of resistance over an 18 year period AID - 10.1101/2021.08.24.457564 DP - 2021 Jan 01 TA - bioRxiv PG - 2021.08.24.457564 4099 - http://biorxiv.org/content/early/2021/08/24/2021.08.24.457564.short 4100 - http://biorxiv.org/content/early/2021/08/24/2021.08.24.457564.full AB - Campylobacter jejuni and Campylobacter coli are important bacterial sources of human foodborne illness. Despite several years of reduced antibiotics usage in livestock production in the UK and US, high prevalence of antimicrobial resistance (AMR) persists in Campylobacter. Both countries have instigated genome sequencing-based surveillance programs for Campylobacter, and here we have identified AMR genes in 32,256 C. jejuni and 8,776 C. coli publicly available genome sequences to compare the prevalence and trends of AMR in Campylobacter isolated in the UK and US between 2001-2018. AMR markers were detected in 68% of C. coli and 53% of C. jejuni, with 15% of C. coli being multi-drug resistant (MDR) compared to only 2% of C. jejuni. The prevalence of aminoglycoside, macrolide, quinolone and tetracycline resistance remained fairly stable from 2001-2018 in both C. jejuni and C. coli, but statistically significant differences were observed between the UK and US. There was a statistically significant higher prevalence of aminoglycoside and tetracycline resistance for US C. coli and C. jejuni, and macrolide resistance for US C. coli. In contrast, UK C. coli and C. jejuni showed a significantly higher prevalence of quinolone resistance. Specific MLST clonal complexes (e.g. ST-353/464) showed &gt;95% quinolone resistance. This large-scale comparison of AMR prevalence has shown that the prevalence of AMR remains stable for Campylobacter in the UK and the US. This suggests that antimicrobial stewardship and restricted antibiotic usage may help contain further expansion of AMR prevalence in Campylobacter, but are unlikely to reduce it in the short term.Competing Interest StatementThe authors have declared no competing interest.