RT Journal Article
SR Electronic
T1 Human IL-10-producing B cells have diverse states induced from multiple B cell subsets
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2021.09.01.458645
DO 10.1101/2021.09.01.458645
A1 Glass, Marla C.
A1 Glass, David R.
A1 Oliveria, John Paul
A1 Mbiribindi, Berenice
A1 Esquivel, Carlos O.
A1 Krams, Sheri M.
A1 Bendall, Sean C.
A1 Martinez, Olivia M.
YR 2021
UL http://biorxiv.org/content/early/2021/09/02/2021.09.01.458645.abstract
AB Regulatory B cells (Bregs) can suppress immune responses through the secretion of IL-10 and other anti-inflammatory cytokines. This immunomodulatory capacity holds therapeutic potential, yet a definitional immunophenotype for enumeration and prospective isolation of B cells capable of IL-10 production remains elusive. We therefore applied mass cytometry to simultaneously quantify cytokine production and immunophenotype in human peripheral B cells across a range of stimulatory conditions and timepoints. While multiple B cell subsets produced IL-10, no phenotype uniquely identified IL-10+ B cells and a significant portion of IL-10+ B cells co-expressed the proinflammatory cytokines IL-6 and TNFα. Despite this heterogeneity, we found operationally tolerant liver transplant recipients had a unique enrichment of IL-10+, but not TNFα+ or IL-6+, B cells as compared to transplant recipients receiving immunosuppression. Thus, human IL-10-producing B cells constitute an induced, transient state arising from a diversity of B cell subsets that may contribute to maintenance of immune homeostasis.Competing Interest StatementThe authors have declared no competing interest.